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Integrated single‐cell landscape of human <t>osteosarcoma.</t> (A) Harmony‐integrated UMAP of 39,063 quality‐controlled cells colored by global cluster. (B) The same embedding colored by patient, showing cross‐patient mixing after integration. (C) Reviewed major‐cell‐type annotations displayed on the integrated UMAP; labels mark lineage centroids. (D) Relative abundance of major cell types in each patient. Colors are consistent between panels C and D.
U2os Human Osteosarcoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human osteosarcoma thymidine kinase deficient cell line 143b  (ATCC)
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(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).
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(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).
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(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).
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human osteosarcoma cell lines saos 2  (ATCC)
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(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).
Human Osteosarcoma Cell Lines Saos 2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human osteosarcoma cell line  (ATCC)
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ATCC human osteosarcoma cell line
(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).
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u2os human osteosarcoma cell line  (ATCC)
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ATCC u2os human osteosarcoma cell line
(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).
U2os Human Osteosarcoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Integrated single‐cell landscape of human osteosarcoma. (A) Harmony‐integrated UMAP of 39,063 quality‐controlled cells colored by global cluster. (B) The same embedding colored by patient, showing cross‐patient mixing after integration. (C) Reviewed major‐cell‐type annotations displayed on the integrated UMAP; labels mark lineage centroids. (D) Relative abundance of major cell types in each patient. Colors are consistent between panels C and D.

Journal: International Journal of Genomics

Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma

doi: 10.1155/ijog/1407947

Figure Lengend Snippet: Integrated single‐cell landscape of human osteosarcoma. (A) Harmony‐integrated UMAP of 39,063 quality‐controlled cells colored by global cluster. (B) The same embedding colored by patient, showing cross‐patient mixing after integration. (C) Reviewed major‐cell‐type annotations displayed on the integrated UMAP; labels mark lineage centroids. (D) Relative abundance of major cell types in each patient. Colors are consistent between panels C and D.

Article Snippet: HOS and U2OS human osteosarcoma cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, United States; HOS, CRL‐1543; U2OS, HTB‐96).

Techniques: Single Cell

TROAP depletion reduces clonogenic growth, overall wound closure, migration, and invasion in osteosarcoma cells. (A) Immunoblot analysis of TROAP in HOS and U2OS cells transfected with siNC, siTROAP‐1, or siTROAP‐2; beta‐actin is the loading control. (B) Colony‐formation images and colony counts. (C) Scratch‐wound images at 0 and 24 h and overall wound closure. Because the assay was not proliferation‐matched, it is not interpreted as direct migration evidence. (D) Transwell migration and invasion images and cell counts. Data are mean + /−standard deviation from three independent biological experiments. One‐way ANOVA with Dunnett′s test compared each siTROAP group with siNC; ∗∗∗ p < 0.001.

Journal: International Journal of Genomics

Article Title: Integrative Single‐Cell and Bulk Transcriptomics Identifies a Proliferative Malignant State and Supports a Functional Contribution of TROAP in Osteosarcoma

doi: 10.1155/ijog/1407947

Figure Lengend Snippet: TROAP depletion reduces clonogenic growth, overall wound closure, migration, and invasion in osteosarcoma cells. (A) Immunoblot analysis of TROAP in HOS and U2OS cells transfected with siNC, siTROAP‐1, or siTROAP‐2; beta‐actin is the loading control. (B) Colony‐formation images and colony counts. (C) Scratch‐wound images at 0 and 24 h and overall wound closure. Because the assay was not proliferation‐matched, it is not interpreted as direct migration evidence. (D) Transwell migration and invasion images and cell counts. Data are mean + /−standard deviation from three independent biological experiments. One‐way ANOVA with Dunnett′s test compared each siTROAP group with siNC; ∗∗∗ p < 0.001.

Article Snippet: HOS and U2OS human osteosarcoma cell lines were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, United States; HOS, CRL‐1543; U2OS, HTB‐96).

Techniques: Migration, Western Blot, Analysis, Transfection, Control, Colony Assay, Standard Deviation

(a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).

Journal: bioRxiv

Article Title: Single-cell variant landscape of the human mitochondrial genome reveals cell type-specific functions and disease mechanisms

doi: 10.64898/2026.10.11.758340

Figure Lengend Snippet: (a) Overview of the study including the development and validation of scMOCHA framework and large-scale discovery workflow for single-cell mtDNA variant analysis in human PBMC and BM samples. (b) Venn diagrams summarize the homoplasmic (purple), heteroplasmic (blue), and somatic (red) variants identified across PBMC and BM datasets. (c) Circos plots illustrate the genomic distribution and allele frequency (AF) of mtDNA variants across the mitochondrial genome in PBMC and BM. Tracks from outer to inner rings are: mitochondrial genome position (black), PhastCons100way conservation (yellow), population AF derived from gnomAD (black), sample frequency of homoplasmic variants (purple) and heteroplasmic variants (blue), the mitochondrial gene annotation map (middle), mean pseudobulk AF of homoplasmic variants (purple), heteroplasmic variants (blue), and somatic variants (red).

Article Snippet: The human osteosarcoma thymidine kinase-deficient cell line 143B(TK − ) (ATCC CRL-8303) and human embryonic kidney HEK293 cells were maintained in Dulbecco’s Modified Eagle Medium GlutaMAX (DMEM; Thermo Fisher Scientific, 10569044) containing high glucose (4.5 g/L) and sodium pyruvate (110 mg/L), supplemented with 1% non-essential amino acids (NEAA) and 10% fetal bovine serum (FBS).

Techniques: Biomarker Discovery, Single Cell, Variant Assay, Analysis, Derivative Assay